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B353 |
WL DIFFERENTIAL BROTH |
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Formula |
B353 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Dextrose Monopotassium phosphate Potassium chloride Calcium chloride Magnesium sulphate Ferric chloride Manganese sulphate Bromo cresol green *Actidione (Cycloheximide) |
5.00 4.00 50.00 0.55 0.425 0.125 0.125 0.0025 0.0025 0.022 0.004 |
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Final pH (at 25°C) : 5.5 + 0.2 |
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Directions |
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Suspend 60.26 gms. of B353 in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate |
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