B352 |
VOGEL JOHNSON AGAR BASE w/o TELLURITE (V.J.AGAR) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Mannitol Dipotassium phosphate Lithium chloride Glycine Phenol red Agar |
10.00 5.00 10.00 5.00 5.00 10.00 0.025 16.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 61 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and add 20 ml of sterile 1% Potassium Tellurite solution. Mix gently and pour into sterile petri plates. |
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