B352 

VOGEL JOHNSON AGAR BASE w/o TELLURITE (V.J.AGAR)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract 

Mannitol 

Dipotassium phosphate 

Lithium chloride 

Glycine 

Phenol red 

Agar 

10.00

5.00

10.00

5.00

5.00

10.00

0.025

16.00

Final pH (at 25°C) : 7.2 + 0.2

Directions

Suspend 61 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and add 20 ml of sterile 1% Potassium Tellurite solution. Mix gently and pour into sterile petri plates.

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