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B350 |
VIOLET RED BILE AGAR |
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Formula |
B350 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Yeast extract Lactose Bile salt mixture Sodium chloride Neutral red Crystal violet Agar |
7.00 3.00 10.00 1.50 5.00 0.03 0.002 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 41.53 gms. of B350 in 1000 ml. distilled water. Heat with stirring to boiling to dissolve the medium completely. DO NOT AUTOCLAVE Cool to 45°C and pour into sterile petri plates containing the inoculum. |
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