B348 |
UREA AGAR BASE (filter sterilizable) (w/o Agar) |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Dextrose Sodium chloride Monopotassium phosphate Phenol red Urea |
1.00 1.00 5.00 2.00 0.012 20.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 29 gms. in 100 ml. distilled water. Mix thoroughly to dissolve completely. Sterilise by filtration. DO NOT BOIL OR AUTOCLAVE. Suspend 15 gm. of agar in 900 ml. of distilled water and dissolve completely by boiling. Sterilise by autoclaving at 15lbs Pressure (121°C) for 15 minutes. Cool to 50-55 °C and mix with 100 ml. of filter sterilized Basal medium. Mix well and aseptically dispense into sterile Tubes to prepare 3 cm. slant and 2 cm. butt. Do not overheat or reheat the medium as urea Decomposes very easily. |
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