B348

UREA AGAR BASE (filter sterilizable) (w/o Agar)

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Dextrose 

Sodium chloride 

Monopotassium phosphate

Phenol red 

Urea 

1.00

1.00

5.00

2.00

0.012

20.00

Final pH (at 25°C) : 6.8 + 0.2

Directions

Suspend 29 gms. in 100 ml. distilled water. Mix thoroughly to dissolve completely. Sterilise by filtration. DO NOT BOIL OR AUTOCLAVE. Suspend 15 gm. of agar in 900 ml. of distilled water and dissolve completely by boiling. Sterilise by autoclaving at 15lbs Pressure (121°C) for 15 minutes. Cool to 50-55 °C and mix with 100 ml. of filter sterilized Basal medium. Mix well and aseptically dispense into sterile Tubes to prepare 3 cm. slant and 2 cm. butt. Do not overheat or reheat the medium as urea Decomposes very easily.

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