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B347I |
UREA AGAR BASE ( CHRISTENSEN ) |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Dextrose Sodium chloride Monopotassium phosphate Phenol red Agar |
1.00 1.00 5.00 2.00 0.012 15.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 24 gms. in 950 ml. distilled water. Boil to dissolve the medium completely. Dispense Into tubes and sterilize by autoclaving at 10lbs Pressure (115°C) for 20 minutes. Cool to 50°C and aseptically add 50 ml of sterile 40% Urea Solution and Mix well . Dispense into sterile Tubes and allow to set in the slanting position. Do not overheat or reheat the medium as urea Decomposes very easily. |
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