B343

TRYPTOSE BLOOD AGAR BASE / W/ YEAST EXTRACT

Formula 

B343

Ingredients 

gms/lit.

Tryptose 

Beef extract 

Yeast extract 

Sodium chloride 

Agar 

10.00

3.00

1.00

5.00

15.00

Final pH (at 25°C) : 

7.3 + 0.2

Directions  

Suspend 34 gms. of B343 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minute For blood agar cool to 45-50°C & aseptically add 7% v/v sterile defibrinated blood. Mix thoroughly, avoiding air bubbles & pour into sterile petri plates.

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