B339I |
TRYPTONE SUCROSE TETRAZOLIUM AGAR BASE (TSTA) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Sodium chloride Saccharose Bile salts Agar |
15.00 5.00 30.00 20.00 0.50 15.00 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 85.5 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 3ml. of 1% Triphenyl Tetrazolium Chloride (TTC) (BF044). Mix well before pouring into sterile petri plates. |
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