B339I

TRYPTONE SUCROSE TETRAZOLIUM AGAR BASE (TSTA)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal 

Sodium chloride

Saccharose 

Bile salts 

Agar 

15.00

5.00

30.00

20.00

0.50

15.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 85.5 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 3ml. of 1% Triphenyl Tetrazolium Chloride (TTC) (BF044). Mix well before pouring into sterile petri plates.

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