B327 

SULPHITE AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium sulphite

Agar

10.00

1.00

20.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 31 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in screw capped tubes containing a clean iron nail in 15 ml. amounts and cap the tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. As an alternate to iron nail, 10 ml. of 5% ferric citrate solution may be used per litre of the medium.

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