B326

SULPHATE API AGAR 

Formula 

B326

Ingredients 

gms/lit.

Yeast extract

Ascorbic acid

Magnesium sulphate

Dipotassium phosphate

Ferrous ammonium sulphate

Sodium chloride

Agar

1.00

0.10

0.20

0.01

0.10

10.00

14.00

Final pH (at 25°C) : 

7.4 + 0.2 

Directions 

Suspend 25.4 gms. of B326 in 1000ml. distilled water. Add 4 ml. of sodium lactate. Heat to dissolve the medium completely. Dispense, preferably in screw –capped tubes in 9 ml. amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes.

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