B325 

STUART TRANSPORT MEDIUM

Formula 

B325 

Ingredients 

gms/lit. 

Sodium thioglycollate

Sodium glycerophosphate 

Calcium chloride 

Methylene blue 

Agar 

1.00

10.00

0.10

0.002

3.00

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 14.1 gms. of B325 in 1000ml. double distilled water. Heat to boiling to dissolve the medium completely. Dispense in to tubes with screw caps to give a depth of approximately 7 cm. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes and after sterilization, tighten the caps. Cool the tubes immediately in an upright position. Care should be taken that the water is free from chlorine.

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