B325 |
STUART TRANSPORT MEDIUM |
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Formula |
B325 |
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Ingredients |
gms/lit. |
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Sodium thioglycollate Sodium glycerophosphate Calcium chloride Methylene blue Agar |
1.00 10.00 0.10 0.002 3.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 14.1 gms. of B325 in 1000ml. double distilled water. Heat to boiling to dissolve the medium completely. Dispense in to tubes with screw caps to give a depth of approximately 7 cm. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes and after sterilization, tighten the caps. Cool the tubes immediately in an upright position. Care should be taken that the water is free from chlorine. |
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