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B315 |
SALT POLYMYXIN BROTH BASE |
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Formula |
B315 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Sodium chloride |
10.00 3.00 20.00 |
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Final pH (at 25°C) |
8.8 + 0.2 |
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Directions |
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Suspend 16.5 gms. of B315 in 500ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°Cand aseptically add rehydrated contents of 1 vial of Polymyxin B Selective Supplement (BF005). Mix well and dispense as desired. |
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