B305 

REINFORCED CLOSTRIDIAL AGAR

Formula 

B305 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Beef extract 

Yeast extract 

Dextrose

Sodium chloride 

Sodium acetate 

Starch,soluble

L-Cysteine hydrochloride

Agar 

10.00 

10.00 

3.00 

5.00

5.00 

3.00 

1.00 

0.50 

13.50 

Final pH (at 25°C) : 6.8 + 0.2.

Directions 

Suspend 51 gms. of B305 in 1000 ml. distilled water . Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes

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