B297 

PHOSPHATE BUFFER, APHA, pH 7.2

Formula

Ingredients 

gms/lit.

Monopotassium phosphate

Sodium carbobate 

26.22

7.78

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 34 gms. of 1000 ml distilled water. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. The pH, after autoclaving and cooling to room
temperature should be 7.2 + 0.2

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