B294

PHENOL RED SUCROSE AGAR

Formula

Ingredients

gms/Lit.

Proteose peptone

Beef extract

Sodium chloride

Sucrose

Phenol red

Agar

10.00

1.00

5.00

10.00

0.025

15.00

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 41 gms. of B294 in 1000 ml distilled water. Heat with frequent agitation to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.  Allow the tubed media to cool in slanted position to form slants with deep butts.

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