B286

PHENOL RED DEXTROSE BROTH 

Formula

Ingredients

gms/lit.

Proteose peptone

Beef extract

Sodium chloride

Dextrose

Phenol red

10.00

1.00

5.00

5.00

0.018

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 21 gms. of B286 in 1000 ml. distilled water Heat to dissolve the medium completely. Dispense in tubes containing inverted Durham’s tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. 

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