B273

NUTRIENT AGAR, pH 6.0 with 0.8% Nacl

Formula 

B273  

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Beef extract 

Sodium chloride 

Agar   

5.00

3.00

8.00

15.00

Final pH (at 25°C) :  

6.0 + 0.2

Directions 

Suspend 31 gms. of B273 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes.

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