B272

NUTRIENT AGAR

Formula 

B272  

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Beef extract 

Sodium chloride 

Agar   

10.00

5.00 

5.00

15.00

Final pH (at 25°C) :  

7.4 + 0.2

Directions 

Suspend 35 gms. of B272 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes.

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