B269

NITRATE AGAR

Formula 

B269 

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Beef extract 

Potassium nitrate  

Agar 

5.00

 3.00

1.00

12.00

Final pH (at 25°C) : 

6.8 + 0.2

Directions

Suspend 21 gms. of B269 in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow to cool the tubes in slanted position.

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