B267

MYCOPLASMA BROTH BASE W/O CV 

(PPLO BROTH BASE W/O SUPPLEMENT W/O CV)

Formula 

B267 

 

Ingredients 

gms/lit. 

Beef heart, infusion from

Peptic digest of animal tissure

Sodium chloride

250.00

10.00

5.00

Final pH (at 25°C) : 7.8 + 0.2

Directions 

Suspend 21 gms.  in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure  (121° C) for 15 minutes. Cool to 45°C and aseptically add 1% Horse serum and Mycoplasma Enrichment Supplement. 

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