B261 |
MOTILITY SULPHIDE MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Beef extract L-Cystine Ferric ammonium citrate Sodium citrate Sodium chloride Gelatin Agar |
10.00 3.00 0.20 0.20 2.00 5.00 80.00 4.00 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 10.44 gms. in 100ml distilled water. Boil with constant agitation to dissolve the medium completely. Dispense in tubes in 4ml amounts and sterilize by autoclaving at 10lbs pressure (115°C) for 15 minutes. Allow the tubed medium to cool in an upright position. |
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