B261 

MOTILITY SULPHIDE MEDIUM

Formula

Ingredients 

gms/lit.

Proteose peptone

Beef extract

L-Cystine

Ferric ammonium citrate

Sodium citrate

Sodium chloride

Gelatin

Agar

10.00

3.00

0.20

0.20

2.00

5.00

80.00

4.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 10.44 gms. in 100ml distilled water. Boil with constant agitation to dissolve the medium completely. Dispense in tubes in 4ml amounts and sterilize by autoclaving at 10lbs pressure (115°C) for 15 minutes. Allow the tubed medium to cool in an upright position.

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