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B232 |
LYSINE IRON AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Yeast extract Dextrose L-Lysine Ferric ammonium citrate Sodium thiosulphate Bromo cresol purple Agar |
5.00 3.00 1.00 10.00 0.50 0.04 0.02 15.00 |
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Final pH (at 25°C) : 6.7 + 0.2 |
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Directions |
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Suspend 34.56 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense Into tubes and sterilize by autoclaving at 15 lbs Pressure (121°C) for 15 minutes. Cool the tubes in slanted position to form slants with deep butts. |
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