B226

LITTMAN OXGALL AGAR

Formula 

B226 

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Diastase

Oxgall

Crystal violet 

Agar

10.00

10.00 

15.00 

0.01 

 20.00 

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 55 gms. of B226 in 1000ml. distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 458C and aseptically add sterile Streptomycin to a final concentration of 30 mcg/ml of medium.

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