B222 |
M-AZIDE BROTH |
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Formula |
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Ingredients |
gm/lit. |
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Tryptone Yeast extract Dextrose Saccharose Dipotassium phosphate Sodium azide |
40.00 10.00 2.00 100.00 4.00 0.40 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 15.64 gm. in 100ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1ml of 1% 2,3,5 Triphenyl Tetrazolium Chloride. Mix well before dispensing. |
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