B216 |
L.S. DIFFERENTIAL MEDIUM |
|
Formula |
||
Ingredients |
gms/lit. |
|
Casein enzymic hydrolysate Papaic digest of soyabean meal Beef extract Yeast extract Dextrose Sodium chloride L-Cysteine hydrochlordie Agar |
10.00 5.00 5.00 5.00 20.00 5.00 0.30 15.00 |
|
Final pH (at 25°C) : 6.1 + 0.2 |
||
Directions |
||
Suspend 65.3 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use ; (1) 100ml of 10% w/v aqueous solution of antibiotic free skim milk powder sterilized at 15 lbs pressure (121°C) for 5 minutes. (2) 10ml of Triphenyl-Tetrazolium Chloride (T.T.C.) Solution. Mix well and pour into sterile petri plates. |
||