B216

L.S. DIFFERENTIAL MEDIUM

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal

Beef extract

Yeast extract

Dextrose

Sodium chloride

L-Cysteine hydrochlordie

Agar

10.00

5.00

5.00

5.00

20.00

5.00

0.30

15.00

Final pH (at 25C) : 6.1 + 0.2

Directions 

Suspend 65.3 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool to 50C and add the following sterile solutions previously kept warm at 50C just prior to use ;

(1) 100ml of 10% w/v aqueous solution of antibiotic free skim milk powder sterilized at 15 lbs pressure (121C) for 5 minutes.

(2) 10ml of Triphenyl-Tetrazolium Chloride (T.T.C.) Solution. Mix well and pour into sterile petri plates.

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