B216

L.S. DIFFERENTIAL MEDIUM

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal

Beef extract

Yeast extract

Dextrose

Sodium chloride

L-Cysteine hydrochlordie

Agar

10.00

5.00

5.00

5.00

20.00

5.00

0.30

15.00

Final pH (at 25°C) : 6.1 + 0.2

Directions 

Suspend 65.3 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use ;

(1) 100ml of 10% w/v aqueous solution of antibiotic free skim milk powder sterilized at 15 lbs pressure (121°C) for 5 minutes.

(2) 10ml of Triphenyl-Tetrazolium Chloride (T.T.C.) Solution. Mix well and pour into sterile petri plates.

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