B214 

KOHN TWO TUBE MEDIUM NO.2

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Casein enzymic hydrolysate 

Sucrose 

Salicin 

Sodium chloride 

Sodium thiosulphate 

Disodium hydrogen orthophosphate 

Bromo thymol blue 

Agar 

10.00

10.00

10.00

10.00

5.00

0.016

0.09

0.02

3.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 48.13 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 10 lbs pressure (115C) for 15 minutes. Cool the tubed medium in an upright position.

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