B213 

KOHN TWO TUBE MEDIUM NO.1

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Yeast extract 

Dextrose

Mannitol 

Phenol red 

Agar 

15.00

2.00

2.00

 1.00

10.00

0.05

16.00

Final pH (at 25C) : 7.2 + 0.2

Directions 

Suspend 46 gms. in 975ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115C) for 15 minutes. Cool to 60C and aseptically add 25 ml of sterile 40% (w/v) Urea solution. Mix well and make slants with a generous butt.

Print This Page

Close Window