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B212 |
KLIGLER IRON AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Yeast extract Proteose peptone Lactose Dextrose Ferrous sulphate Sodium chloride Sodium thiosulphate Phenol red Agar |
15.00 3.00 3.00 5.00 10.00 1.00 0.20 5.00 0.30 0.024 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 57.5 gms. in 1000 ml. distilled water. Mix well & distribute into tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes to set as slopes with 1 inch butts. |
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