B212 

KLIGLER IRON AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Yeast extract 

Proteose peptone 

Lactose 

Dextrose 

Ferrous sulphate 

Sodium chloride 

Sodium thiosulphate

Phenol red 

Agar 

15.00

3.00

3.00

5.00

10.00

1.00

0.20

5.00

 0.30

0.024

15.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 57.5 gms. in 1000 ml. distilled water. Mix well & distribute into tubes. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool the tubes to set as slopes with 1 inch butts.

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