B208 

IRON SULPHITE AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium sulphite

Iron (III) citrate

Agar

10.00

0.50

0.50

15.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 26 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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