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B188 |
SPIRIT BLUE AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Spirit blue Agar |
10.00 5.00 0.15 17.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 32.15 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15minutes. Cool to 50°C & add 30 ml of lipase substrate slowly while agitating to obtain an even |
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