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B187 |
ENTEROCOCCUS CONFIRMATORY BROTH |
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Formula |
B187 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Dextrose Sodium azide Methylene blue Sodium chloride |
5.00 5.00 5.00 0.40 0.01 65.00 |
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Final pH (at 25°C) |
8.0 + 0.2 |
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Directions |
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Suspend 80.4 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in 100 ml quantities in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the agar tubes to cool in a slanted position and cool the broth to room temperature and add 65 units of Penicillin to each 100 ml of broth prior to use. |
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