B181 |
EDWARDS MEDIUM, MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Esculin Sodium chloride Thallous sulphate Crystal violet Agar |
10.00 10.00 1.00 5.00 0.33 0.0013 15.00 |
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Final pH (at 25°C) :7.4 + 0.2 |
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Directions |
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Suspend 41.33 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Cool to 50°C & aseptically add 5 to 7% v/v sterile bovine or sheep blood. Mix well and pour into sterile petri plates. |
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