B181 

EDWARDS MEDIUM, MODIFIED

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Esculin 

Sodium chloride 

Thallous sulphate 

Crystal violet 

Agar 

10.00

10.00

1.00

5.00

0.33

0.0013

15.00

Final pH (at 25°C) :7.4 + 0.2

Directions 

Suspend 41.33 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Cool to 50°C & aseptically add 5 to 7% v/v sterile bovine or sheep blood. Mix well and pour into sterile petri plates.

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