B165

DEXTROSE AGAR 

Formula 

B165 

Ingredients 

gms/lit.

Tryptose 

Beef extract 

Dextrose 

Sodium chloride 

Agar 

10.00

3.00

10.00

5.00

15.00

Final pH (at 25°C)

7.3 + 0.2

Directions 

Suspend 43 gms. of B165 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired Blood agar can be prepared by the addition of 5% v/v sterile, defibrinated sheep blood into sterile Dextrose Agar, cooled to 50°C. Mix well and dispense as desired.

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