B165 |
DEXTROSE AGAR |
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Formula |
B165 |
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Ingredients |
gms/lit. |
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Tryptose Beef extract Dextrose Sodium chloride Agar |
10.00 3.00 10.00 5.00 15.00 |
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Final pH (at 25°C) |
7.3 + 0.2 |
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Directions |
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Suspend 43 gms. of B165 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired Blood agar can be prepared by the addition of 5% v/v sterile, defibrinated sheep blood into sterile Dextrose Agar, cooled to 50°C. Mix well and dispense as desired. |
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