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B161 |
MOELLER DECARBOXYLASE BROTH BASE |
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Formula |
B161 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Dextrose Bromo cresol purple Cresol red Pyridoxal |
5.00 5.00 0.50 0.01 0.005 0.005 |
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Final pH (at 25°C) : 6.0 + 0.2 |
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Directions |
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Suspend 10.52 gms. of (B161) in 1000 ml. distilled water. Add 10gm. of L-Lysine, LArginine, L-Ornithine or other L-amino acids. When using DL-amino acids., use 2% concentration. Heat to dissolve the medium completely. When L-Ornithine is added, readjustment of the pH is required. Dispense in 5ml. amount in screw-capped tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. |
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