B160 

DERMATOPHYTE TEST AGAR BASE (D. T. M. AGAR)

Formula

Ingredients 

gms/lit.

Papaic digest of soyabean meal 

Glucose 

Phenol red 

Agar 

10.00

10.00

0.20

20.00

Final pH (at 25°C) : 5.5 + 0.2

Directions 

Suspend 20.1 gms. in 500 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Aseptically add the rehydrated contents of one vial of D.T.M. Supplement. Mix well before pouring into sterile petri plates.

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