B155 |
CYSTINE TRYPTONE AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate L-Cystine Sodium chloride Sodium sulphite Phenol red Agar |
20.00 0.50 5.00 0.50 0.017 2.50 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 28.5 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Dispense in tubes in 8 – 10 ml amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add appropriate arbohydrate. Mix well and allow the tubed medium to cool in an upright position. |
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