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B154 |
CYSTINE HEART AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Beef heart infusion from Proteose peptone Dextrose Sodium chloride L-cystine Agar |
500.00 10.00 10.00 5.00 1.00 15.00 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 51 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. When to be enriched with haemoglobin, suspend 10.2 gms. in 100 ml distilled water. Sterilize as above. Cool to 50°C and aseptically add 100 ml of 2% sterile haemoglobin solution.Mix well and pour into sterile petri plates. |
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