B144 

BI.G.G.Y. AGAR (NICKERSON AGAR)

Formula

Ingredients 

gms/lit.

Yeast extract

Glycine 

Dextrose 

Bismuth ammonium citrate 

Sodium sulphite 

Agar 

1.00

10.00

10.00

5.00

3.00

16.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 45 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEATING. Overheating will destroy the selective properties. Disperse the flocculant precipitate formed by swirling prior to dispensing into plates.

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