B134 |
CHO MEDIUM BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enymic hydrolysate Yeast extract L-cystine Sodium chloride Ascorbic acid Sodium thioglycollate Bromo thymol blue Agar |
15.00 7.00 0.25 2.50 0.10 0.50 0.01 0.75 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 26 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C ) for 15 minutes. Cool to 45- 50°C. Aseptically add 6.25 ml of 10% sterile carbohydrate solution. Mix well and dispense in sterile tubes containing inverted Durham’s tubes. |
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