B133

C.L.E.D. AGAR (WITH BROMO THYMOL BLUE)

Formula 

B133 

Ingredients 

gms/lit. 

Peptic digest of animal tissue 

Casein enzymic hydrolysate 

Beef extract 

Lactose 

L-cystine 

Bromo thymol blue 

Agar 

4.00

4.00 

3.00 

10.00 

0.128 

0.02

15.00 

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 36.15 gms. of B133 in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Print This Page

Close Window