B133 |
C.L.E.D. AGAR (WITH BROMO THYMOL BLUE) |
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Formula |
B133 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Casein enzymic hydrolysate Beef extract Lactose L-cystine Bromo thymol blue Agar |
4.00 4.00 3.00 10.00 0.128 0.02 15.00 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 36.15 gms. of B133 in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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