B132 

C.L.E.D. AGAR (W/ANDRADE INDICATOR)

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Casein enzymic hydrolysate 

Beef extract 

Lactose 

L-Cystine 

Bromo thymol blue 

Andrade indicator 

Agar 

4.00

4.00

3.00

10.00

0.128

0.02

0.10

15.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 36.25 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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