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B124 |
BRILLIANT GREEN AGAR, MODIFIED WITH 1.2% AGAR |
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Formula |
B124 |
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Ingredients |
gms/lit. |
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Proteose peptone Yeast extract Lactose Sucrose Sodium chloride Phenol red Brilliant green Agar |
10.00 3.00 10.00 10.00 5.00 0.08 0.0125 12.00 |
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Final pH (at 25°C) : 6.9 + 0.2 |
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Directions |
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Suspend 50 gms. of B124 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. For more selectivity, aseptically add rehydrated Sulpha supplement. Mix well before pouring into sterile petri plates. |
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