B1211 

M-CP AGAR BASE

Formula

Ingredients 

gms/lit.

Tryptose

Yeast extract

Sucrose

L-Cystine hydrochloride

Magnesium sulphate,7H2O

Ferric chloride, 6 H2O

Bromo cresol purple

Indoxyl-β-D-glucoside

Agar

30.00

20.00

5.00

1.00

0.10

0.09

0.04

0.06

15.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 35.60 gms. dehydrated powder in 485ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Aseptically add the rehydrated contents of 1 vial of M-CP Selective Supplement – I and 1 vial of M-CP Selective Supplement – II. Mix well and pour into sterile petri plates.

Print This Page

Close Window