B121

BRAIN HEART INFUSION BROTH

Formula 

B121 

Ingredients 

gms/lit.

Calf brain,infusion from 

Beef infusion from 

Proteose peptone 

Dextrose

Sodium chloride 

Disodium phosphate 

200.00

250.00

10.00 

2.00

5.00

2.50

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 37 gms. of B121 in 1000 ml. distilled water. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

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