B1178 

ANAEROBIC BLOOD AGAR BASE 

Formula 

Ingredients 

gms/lit.

Papaic digest of soyabean meal

Casein enzymic hydrolysate 

Growth factors 

Sodium chloride 

Agar  

5.00

14.50

1.50

5.00

14.00

Final pH (at 25°C) : 7.0 ± 0.2 

Directions 

Suspend 40 gms. in 990 ml. distilled water. Boil gently to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of one vial of Neomycin supplement (BF106) and 5% v/v defibrinated sheep blood. Mix thoroughly before pouring into sterile plates.

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