B1145

KANAMYCIN ESCULIN AZIDE BROTH BASE

Formula 

B1145 

Ingredients 

gms/lit. 

Tryptone

Yeast extract 

Sodium chloride 

Sodium citrate 

Esculin 

Ferric ammonium citrate

Sodium azide

 

20.00 

5.00 

5.00 

1.00 

1.00 

 0.50 

 0.15 

Final pH (at 25C) : 7.0 + 0.2

Directions 

Suspend 32.62gms. of B1145 in 1000ml. distilled water. Add one vial of Kanamycin sulphate selective supplement reconstituted as directed. Bring to boil. Dispense into final containers & Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes.

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