|
B114 |
B.G. SULPHA AGAR (BRILLIANT GREEN SULPHA AGAR) |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Yeast extract Proteose peptone Lactose Sucrose Sodium sulphapyridine Sodium chloride Brilliant green Phenol red Agar |
3.00 10.00 10.00 10.00 1.00 5.00 0.0125 0.08 20.00 |
|
|
Final pH (at 25°C) : 6.9 + 0.2 |
||
|
Directions |
||
|
Suspend 59 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. To maintain selectively of the medium, DO NOT OVER STERILIZE OR OVERHEAT the medium. |
||