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B1095 |
L.D. EGG YOLK AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Sodium chloride Sodium sulphite L-Cystine L-Tryptophan Dextrose Disodium phosphate Magnesium sulphate Hemin Vitamin K1 Agar |
5.00 5.00 2.50 0.10 0.40 0.20 2.00 5.00 0.01 0.01 0.01 20.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 40.23 gms. in 900ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 60°C and aseptically add 100 ml sterile Egg Yolk Emulsion (BF003). Mix well and pour into sterile petri plates. |
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