B1094 

L.D. ESCULIN AGAR

Formula

Ingredients 

gms/lit.

Tryptose 

Yeast extract 

Sodium chloride 

L-Tryptophan 

Vitamin K1 

L-Cystine 

Hemin 

Esculin 

Ferric citrate 

Agar 

5.00

5.00

2.50

0.20

0.01

0.40

0.01

1.00

0.50

20.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 34.62 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. 

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