B1093 

L.D. AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolyate

Yeast extract 

Sodium chloride 

Sodium sulphite 

L-Cystine 

L-Tryptophan 

Vitamin K1 

Hemin 

Agar

5.00

5.00

2.50

0.10

0.40

0.20

0.01

0.01

 20.00

Final pH (at 25C) : 7.4 + 0.2

Directions 

Suspend 33.22 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes.

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