B1091 |
KUPFERBERG TRICHOMONAS BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Maltose L-cysteine hydrochloride Methylene blue Agar |
20.00 1.00 1.50 0.003 1.00 |
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Final pH (at 25°C) : 6.0 + 0.2 |
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Directions |
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Suspend 23.5 gms. in 950ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add 50 ml sterile beef or human serum and rehydrated contents of Trichomonas Selective Supplement (BF069) or 1 mg Chloramphenicol per ml of medium. Mix well and dispense as desired. |
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