B1091 

KUPFERBERG TRICHOMONAS BROTH BASE

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Maltose 

L-cysteine hydrochloride 

Methylene blue

Agar 

20.00

1.00

1.50

0.003

1.00

Final pH (at 25C) : 6.0 + 0.2

Directions 

Suspend 23.5 gms. in 950ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool to 50-55C and aseptically add 50 ml sterile beef or human serum and rehydrated contents of Trichomonas Selective Supplement (BF069) or 1 mg Chloramphenicol per ml of medium. Mix well and dispense as desired.

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